Changes in canine position during maxillary molar distalization with mini-implant and CD appliance: an in vitro study / 口腔疾病防治

Objective@#To investigate changes in the three⁃dimensional position of the maxillary canine during the distal movement of the maxillary first molar by a mini⁃implant combined with a CD appliance.@*Methods@#Ten typodont models of class II malocclusion were selected, and one side was randomly chosen as the experimental group. The CD ap⁃ pliance was bonded to the maxillary canine and first molar of the experimental group, and 1.2 mm stainless steel wire was bent as the anchorage, which was fixed on the model to simulate mini⁃implants implanted in the zygomatic alveolar ridge. Then, 180 g orthodontic force was applied to the canine of the experimental group; the other side was recognized as the control group and was not used for strengthening. Six mini⁃implants were implanted in different parts of the mod⁃ el and used as a reference before and after the experiment. The models were placed in an incubator and heated at 56 ℃ in a water bath for 2 min. The models were scanned before and after thermostatic water bath treatment with a 3⁃shape scanner. Then, the digital models were overlapped through the reference points, and the positions of the canines and first molars were measured before and after the experiment.@*Results@#The sagittal movement distance of the first molar in the experimental group was ( 0.25 ± 0.33) mm, and the vertical movement distance was (0.25 ± 0.28 )mm, which was significantly different from the control group (P < 0.05), while the transverse change was not significantly different (P > 0.05). There was no significant difference in the root position of the first molar in the experimental group (P > 0.05). The lateral and vertical displacement distances of the maxillary canine crown in the experimental group were (4.03 ± 2.11) mm and (1.86 ± 1.01) mm, respectively, which were significantly different from those in the control group (P < 0.001), while the sagittal changes showed no significant differences (P > 0.05). In the experimental group, there was no significant difference in the position of the apex of the maxillary cusp (P > 0.05).@*Conclusion@#Our in vitro study showed that the maxillary canines inclined buccally accompanied by a small amount of intrusion during molar distaliza⁃ tion by a mini⁃implant combined with a CD appliance.

Draft genome analysis of Dietzia sp. 111N12-1, isolated from the South China Sea with bioremediation activity

Abstract Dietzia sp. 111N12-1, isolated from the seawater of South China Sea, shows strong petroleum hydrocarbons degradation activity. Here, we report the draft sequence of approximately 3.7-Mbp genome of this strain. To the best of our knowledge, this is the first genome sequence of Dietzia strain isolated from the sea. The genome sequence may provide fundamental molecular information on elucidating the metabolic pathway of hydrocarbons degradation in this strain.

A SNP in 5′ untranslated region of CD40 gene is associated with an increased risk of ischemic stroke in a Chinese population: a case-control study

Abstract Cluster of differentiation 40 (CD40), the receptor for CD154, is a member of the tumor necrosis factor (TNF) receptor superfamily. Several studies have been conducted to investigate the effect of the CD40 rs1883832 polymorphism on atherosclerotic disease in different population; however, inconsistent results were obtained. In this study, we investigated the association of four polymorphisms (rs1883832, rs13040307, rs752118 and rs3765459) of CD40 gene and their effect on CD40 expression with the risk of ischemic stroke (IS) in a Chinese population. Three hundred and eighty patients with IS and 450 control subjects were included in the study. The CD40 polymorphisms were discriminated by Snapshot SNP genotyping assay. Serum soluble CD40 (sCD40) levels were detected by ELISA. We found that the rs1883832CT and rs1883832TT genotypes were associated with an increased risk of IS compared with the rs1883832CC genotype (OR = 1.42, 95% CI: 1.03-1.95, p = 0.030 and OR = 1.91, 95% CI: 1.29-2.82, P = 0.001, respectively), and the rs1883832T allele was associated with a significantly increased risk of IS compared with rs1883832C allele (OR = 1.40, 95% CI: 1.15-1.70, P = 0.001). Elevated serum sCD40 levels were observed in patients with IS compared with the control gropu (P < 0.01). Individuals carrying the rs1883832TT or rs1883832CT genotypes showed significantly higher sCD40 levels compared with the rs1883832CC genotype in the IS group [(64.8 ± 25.4 pg/mL, TT = 94); (63.9 ± 24.3 pg/mL, CT = 185) vs (53.3 ± 22.5 pg/mL, CC = 101), P < 0.01]. The TCCA haplotype was associated with an increased risk of IS compared with the control group (OR = 2.10, 95% CI: 1.23-3.58, p = 0.005). However, we did not find a significant association between the other three polymorphisms and IS risk. In conclusion, after a comprehensive comparison with other studies, we confirmed that the rs1883832T allele but not the rs1883832C allele is associated with an increased risk of IS. The rs1883832 polymorphism may exert influences on abnormal CD40 expression in IS patients among the Chinese population.